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KMID : 0545120030130010035
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Journal of Microbiology and Biotechnology
2003 Volume.13 No. 1 p.35 ~ p.42
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Cloning and Characterization of a Bifunctional Cellulase-Chitosanase Gene from Bacillus licheniformis NBL420
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HONG, IN-PYO
JANG, HONG-KI/LEE, SHIN-YOUNG/CHOI, SHIN-GEON
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Abstract
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A 1.3 kb cellulase gene encoding novel bifunctional cellulase-chitosanase activity was cloned from biopolymer-producing alkali-tolerant B. licheniformis NBL420 in E. coli. A recombinant cellulase-chitosanase, named CelA, was expressed and purified to homogeneity. The activity staining and the enzymatic characterization of the purified CelA revealed bifunctional activities on carboxymethyl cellulase (CMC) and glycol-chitosan. The similar characteristics of the enzymatic activities at the optimum pH, optimum temperature, and thermostability indicated that CelA used a common catalytic domain with relaxed substrate specificity. A comparison of the deduced amino acids in the N-terminal region revealed that the mature CelA had a high homology with the previously identified bifunctional cellulase-chitosanase of Myxobacter sp. AL-1.
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